Biomolecular kinetics : a step-by-step guide
AUTHOR : Bagshaw, C. R. (Clive Richard)
CALL NO : QU34 B149b 2017
IMPRINT : Boca Raton, Fla. : CRC Press, c2017
This illustrated treatment explains the methods used for measuring how much a reaction gets speeded up, as well as the framework for solving problems such as ligand binding and macromolecular folding, using the step-by-step approach of numerical integration. It is a thoroughly modern text, reflecting the recent ability to observe reactions at the single-molecule level, as well as advances in microfluidics which have given rise to femtoscale studies. Kinetics is more important now than ever, and this book is a vibrant and approachable entry for anyone who wants to understand mechanism using transient or single molecule kinetics without getting bogged down in advanced mathematics.
Features
- Worked answers to exercises in the book are available to confirmed adopting instructors.
- Introduces the analytical rate equations for basic one and two-step reactions relevant to the interactions of macromolecules with ligands.
- Extends the analysis using the step-by-step approach of numerical integration and the “dice-rolling” approach of stochastic simulations.
- Discusses the theory and practice of elucidating biomolecular mechanisms, including enzyme action, protein folding, protein-nucleic acid interactions and molecular motors.
- Provides a comprehensive description of instruments and techniques for following transient reactions on a sub-second time scale and at the single-molecule level.
- Illustrates the analysis of reaction records using computer simulation and fitting, using commercial software and freeware.
Source : www.crcpress.com/Biomolecular-Kinetics-A-Step-by-Step-Guide/Bagshaw/p/book/9781498727235
