Single-molecule studies of nucleic acids and their proteins
AUTHOR : –
CALL NO : QU58 S617 2019
IMPRINT : Oxford : Oxford University Press, c2019
This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.
- Lucidly explains the methods for single molecule manipulations
- Clearly describes the basis for fluorescence studies (lifetimes, rates, FRET, FCS, super-resolution microscopy, etc.) and their pitfalls
- Discusses the various polymer models (FJC, WLC, RLC, etc.) and their relevance to the physical properties of Nucleic acids
- Describes the use of the various methods for the study of three essential DNA (and RNA) processing enzymatic families (polymerases, helicases and topoisomerases)
